Scientist II, Protein Expression and Purification

About this role

Scientist II, Protein Expression and Purification Location: San Diego, CA Job Type: Full-Time

Compensation: $110,000 to $120,000

About Us

We are a venture-backed, stealth-stage biotechnology company based in San Diego, focused on developing novel technologies that will redefine how disease is detected, characterized, and managed with a novel approach to clinical genomics. Our mission is to fundamentally transform healthcare through a convergence of innovation across multiple scientific disciplines.

Founded by industry veterans with decades of experience in life sciences tools and diagnostics, our leadership team brings a proven track record of translating scientific insight into successful commercial products. Our investors include some of the most respected names in healthcare and deep tech.

Position Overview

We are seeking a Scientist II, Enzyme Assay & Characterization to play a key role in developing and executing biochemical assays that support the company’s multi-omics platform development. This individual will optimize and implement assays.

The Scientist II will contribute directly to enzyme evaluation. This role offers opportunities for broad technical growth, including assay development, molecular biology, protein engineering and integration.

The candidate must be detail-oriented, analytical, and collaborative, thriving in a dynamic environment that values innovation, cross-disciplinary interaction, and scientific rigor.

Key Responsibilities

· Clone, express, and screen protein constructs in bacterial expression systems.

· Optimize bacterial expression conditions.

· Execute purification workflows.

· Characterize protein quality.

· Optimize and implement biochemical assays.

· Develop, optimize, and validate spectroscopic and fluorescence-based assays.

· Troubleshoot and refine assays for reproducibility, sensitivity, and throughput.

· Collaborate closely with protein expression/purification, enzymology, protein engineering, and molecular biology teams.

· Document methods, maintain rigorous records, contribute to SOPs and prepare data summaries and reports.

· Support lab organization, maintain equipment, and ensure compliance with safety and reproducibility practices.

Qualifications Required

· B.S. in Biochemistry, Molecular Biology, Enzymology, or related field with 4–6 years of relevant experience, OR M.S. with 2–4 years of relevant experience.

· Demonstrated experience optimizing and implementing biochemical assays.

· Hands-on experience with spectroscopic and fluorescence-based assays.

· Hands-on experience with gel electrophoresis and gel-based assays.

· Strong experience with data visualization software.

· Excellent organizational, problem-solving, and communication skills.

· Ability to work independently and collaboratively in cross-functional teams.

Why Join Us

· Influence the foundational technologies and strategies of a company poised to shape the future of clinical genomics and healthcare.

· Work in a dynamic, collaborative environment where innovation and scientific rigor are deeply valued.

· Join a seasoned and multidisciplinary team tackling high-impact problems at the intersection of science and engineering.

· Competitive compensation and equity package, comprehensive benefits, and flexibility to support work-life integration.

We are an equal opportunity employer. We thrive on diversity and collaboration.

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